GeneTex
  • Country / Location Selection

United States (US)

Influenza B virus Nucleoprotein antibody

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in Western Blot (WB). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539
Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128539

Cat. No. GTX128539

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Influenza B virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629882 / GTX629883, Detection : GTX128539 or Capture : GTX128539, Detection : GTX629882

Not tested in other applications.

Positive Control

Influenza B virus (Taiwan B70555) infected medium

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant fragment corresponding to C-terminal region of Influenza B virus NP (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885797

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Research Area

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539

GTX128539 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody (GTX128539) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody used in Western Blot (WB). GTX128539

GTX128539 WB Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by Western blot analysis.
A. 20 μl non infected medium
B. 20 μl Influenza B virus (Taiwan B70555) infected medium
7.5 % SDS-PAGE
Influenza B Virus NP antibody (GTX128539) dilution: 1:100000

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539

GTX128539 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128539

GTX128539 ICC/IF Image

Influenza B virus Nucleoprotein antibody detects Influenza B virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX128539) diluted at 1:2000.

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128539

GTX128539 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: MDCK cells infected with Influenza B virus (Taiwan B70555).
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128539) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Influenza B virus Nucleoprotein antibody Cat. No. GTX128539

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27389476
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169