Influenza B virus Nucleoprotein antibody

Name: Influenza B virus Nucleoprotein antibody
Brand: GeneTex
SKU: GTX128539
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128539

Anti-Influenza B virus Nucleoprotein antibody used in Western Blot (WB). GTX128539

Anti-Influenza B virus Nucleoprotein antibody used in ELISA (ELISA). GTX128539

Cat No. GTX128539

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA, Sandwich ELISA
Reactivity	Influenza B virus
Package	100 μl ($329), 25 μl ($169)

See all Influenza B virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5000-1:100000
ICC/IF	1:100-1:1000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX629882/GTX629883, Detection: GTX128539 or Capture:GTX128539 , Detection: GTX629882

Not tested in other applications.

Positive Control

virus infected medium (Taiwan B70555)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza B Virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885797

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Research Area

DATA IMAGES

GTX128539 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: MDCK cells infected with Influenza B virus (Taiwan B70555).
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128539) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128539 WB Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by Western blot analysis.
A. 20 μl non infected medium
B. 20 μl Influenza B virus (Taiwan B70555) infected medium
7.5 % SDS-PAGE
Influenza B Virus NP antibody (GTX128539) dilution: 1:100000

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody (GTX128539) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128539 ICC/IF Image

Influenza B virus Nucleoprotein antibody detects Influenza B virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX128539) diluted at 1:2000.

REFERENCE

Application Reference

Sakai K et al. Sci Rep 2016; 6 : 29430 TMPRSS2 Independency for Haemagglutinin Cleavage In Vivo Differentiates Influenza B Virus from Influenza A Virus.

Application : WB

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REVIEW