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Influenza B virus Nucleoprotein antibody

Anti-Influenza B virus Nucleoprotein antibody used in Western Blot (WB). GTX135819
Anti-Influenza B virus Nucleoprotein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135819
Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135819

Cat. No. GTX135819

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-P (cell pellet)

Reactivity

 Influenza B virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza B virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:110000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza B virus Nucleoprotein (B/Victoria/02/1987). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909885

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody used in Western Blot (WB). GTX135819

GTX135819 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody (GTX135819) diluted at 1:110000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135819

GTX135819 IHC-P (cell pellet) Image

Influenza B virus Nucleoprotein antibody detects Influenza B virus Nucleoprotein protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and Influenza B virus Nucleoprotein transfected 293T cell FFPE Cell Pellet Block.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX135819) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Influenza B virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135819

GTX135819 ICC/IF Image

Influenza B virus Nucleoprotein antibody detects Influenza B virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX135819) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Myers ML et al. Front Immunol 2022; 13 : 1002286 Impact of adjuvant: Trivalent vaccine with quadrivalent-like protection against heterologous Yamagata-lineage influenza B virus.
Application : IHC-P
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169