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Inhibin beta A antibody

Orthogonal
Anti-Inhibin beta A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108405
Anti-Inhibin beta A antibody used in IHC (Paraffin sections) (IHC-P). GTX108405
Anti-Inhibin beta A antibody used in Western Blot (WB). GTX108405
Anti-Inhibin beta A antibody used in Western Blot (WB). GTX108405

Cat. No. GTX108405

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Inhibin beta A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Inhibin beta A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950590

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibin subunit beta A , EDF , FRP

Cellular Localization

Secreted

Background

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Inhibin beta A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108405

GTX108405 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A (GTX108405) antibody at 1:200 dilution.

Anti-Inhibin beta A antibody used in IHC (Paraffin sections) (IHC-P). GTX108405

GTX108405 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CLEAR CELL OVCA xenograft, using Inhibin beta A(GTX108405) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Inhibin beta A antibody used in Western Blot (WB). GTX108405

GTX108405 WB Image

Orthogonal

PC-3 conditioned medium (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody (GTX108405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Inhibin beta A antibody used in Western Blot (WB). GTX108405

GTX108405 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Inhibin beta A antibody (GTX108405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hu CM et al. Adv Sci (Weinh) 2023; 10 (36) : e2301240 Oncogenic KRAS, Mucin 4, and Activin A-Mediated Fibroblast Activation Cooperate for PanIN Initiation.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169