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Ins antibody

Anti-Ins antibody used in IHC (Whole mount) (IHC-Wm). GTX128490
Anti-Ins antibody used in IHC (Whole mount) (IHC-Wm). GTX128490

Cat. No. GTX128490

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Ins products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Ins. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885787

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

preproinsulin , zgc:109842

Cellular Localization

Secreted

Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Database

Research Area

DATA IMAGES

Anti-Ins antibody used in IHC (Whole mount) (IHC-Wm). GTX128490

GTX128490 IHC-Wm Image

Ins antibody detects Ins antibody protein at endocrine β-cells on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Ins antibody (GTX128490) dilution: 1:200.
Anti-Ins antibody used in IHC (Whole mount) (IHC-Wm). GTX128490

GTX128490 IHC-Wm Image

Ins antibody detects Ins protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 3 days-post-fertilization zebrafish embryo.
Green: Ins stained by Ins antibody (GTX128490) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

Dong G et al. Genesis 2019; : e23345 Mypt1 regulates Bmp signaling to promote embryonic exocrine pancreas growth in zebrafish.
Reactivity : Zebrafish
Zhang YM et al. Dev Cell 2018; 45 (5) : 580-594.e7 distant * signaling regulates vertebrate pigmentation through the sheddase bace2.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169