50 mM sodium phosphate buffer, 0.2% gelatin containing =0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Partially purified placental insulin receptor
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The insulin receptor is a tetrameric transmembranous glycoprotein composed of two alpha subunits (120kD) and two beta subunits (90kD). The alpha subunits are disulfide linked, totally extracellular and are the insulin binding site. The beta subunits are transmembranous and contain an intracellular tyrosine kinase domain.The biological effects of insulin are mediated primarily through the insulin receptor. The interaction of insulin with the alpha-subunit of the insulin receptor activates the protein tyrosine kinase of the beta-subunit which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adaptor proteins, IRS-1, IRS-2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins.