GeneTex
United States (US)

Insulin Receptor antibody [29B4]

Cat No. GTX16884

Host Mouse
Clonality Monoclonal
Clone Name 29B4
Isotype IgG1
Application IP, Neut
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For IP: Use at a concentration of 1-2 μg/sample. For Neut: Use at an assay dependent dilution. Not suitable for WB nor IHC-P. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

156 kDa. ( Note )

Positive Control

HepG2 cells
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer, 0.2% gelatin containing =0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Partially purified placental insulin receptor

Purification

Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Receptor,Cd220,Hhf5,Insr

Background

The insulin receptor is a tetrameric transmembranous glycoprotein composed of two alpha subunits (120kD) and two beta subunits (90kD). The alpha subunits are disulfide linked, totally extracellular and are the insulin binding site. The beta subunits are transmembranous and contain an intracellular tyrosine kinase domain.The biological effects of insulin are mediated primarily through the insulin receptor. The interaction of insulin with the alpha-subunit of the insulin receptor activates the protein tyrosine kinase of the beta-subunit which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adaptor proteins, IRS-1, IRS-2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins.

Database

Research Area

REFERENCE
Package List Price ($)
$ 319