Insulin Receptor beta antibody

Name: Insulin Receptor beta antibody
Brand: GeneTex
SKU: GTX101136
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

Anti-Insulin Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101136

Anti-Insulin Receptor beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101136

Cat. No. GTX101136

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all Insulin Receptor products

View product citations for antibody GTX101136 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

NIH-3T3 , PC-12 , rat liver

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Insulin Receptor beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3643 INSR
UniProt: P06213 INSR

Research Area

DATA IMAGES

GTX101136 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136 IHC-P Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Insulin Receptor beta antibody (GTX101136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101136 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136 IHC-P Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Insulin Receptor beta antibody (GTX101136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101136 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX101136 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136 WB Image

Various whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor Beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX101136 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor Beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136 ICC/IF Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein at cytoplasm and cell membrane by immunofluorescent analysis.
Sample: RT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Insulin Receptor stained by Insulin Receptor beta antibody (GTX101136) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101136 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136 WB Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) at a dilution of 1:1000.

REFERENCE

Application Reference

Jafari-Rastegar N et al. J Diabetes Metab Disord 2023; 22 (1) : 297-305 Oral administration of nano-tyrosol reversed the diabetes-induced liver damage in streptozotocin-induced diabetic rats.

Tucker TR et al. Dis Model Mech 2023; 16 (8) An inducible model of chronic hyperglycemia.

Su Y et al. Front Pharmacol 2023; 14 : 1115387 Radix Rehmanniae Praeparata (Shu Dihuang) exerts neuroprotective effects on ICV-STZ-induced Alzheimer's disease mice through modulation of INSR/IRS-1/AKT/GSK-3β signaling pathway and intestinal microbiota.

Application : WB

Reactivity : Mouse

Dominici FP et al. Br J Pharmacol 2020; 177 (20) : 4766-4781 Activation of AT2 receptors prevents diabetic complications in female db/db mice by NO-mediated mechanisms.

Application : WB

Reactivity : Mouse

Huang TW et al. Acta Biomater 2019; (Epub) Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2.

Application : WB

Reactivity : Human

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