GeneTex
United States (US)

Insulin Receptor antibody

GTX101136_42690_20170706_WB_R_liver_19011506_736.jpg
GTX101136_41059_IHC_M_19011506_757.jpg
GTX101136_42690_20170223_WB_19011506_549.jpg
GTX101136_39932_WB_M_19011506_801.jpg
GTX101136_39932_IHC_19011506_483.jpg
GTX101136_42690_20170706_WB_R_19011506_760.jpg

Cat No. GTX101136

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

293T , NIH-3T3 , PC-12 , rat liver
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Insulin Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD220 antibody, HHF5 antibody, INSR antibody, IR antibody, insulin receptor antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101136_42690_20170706_WB_R_liver_19011506_736.jpg

GTX101136 WB Image

Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136_41059_IHC_M_19011506_757.jpg

GTX101136 IHC-P Image

Insulin Receptor antibody detects Insulin Receptor protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Insulin Receptor antibody (GTX101136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101136_42690_20170223_WB_19011506_549.jpg

GTX101136 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor antibody (GTX101136) diluted at 1:1000.

GTX101136_39932_WB_M_19011506_801.jpg

GTX101136 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX101136 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101136_39932_IHC_19011506_483.jpg

GTX101136 IHC-P Image

Insulin Receptor antibody detects INSR protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Insulin Receptor antibody (GTX101136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101136_42690_20170706_WB_R_19011506_760.jpg

GTX101136 WB Image

Various whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Insulin Receptor antibody Cat No. GTX101136

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169