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Insulin Receptor beta antibody [HL2737]

Recombinant antibody Orthogonal
Anti-Insulin Receptor beta antibody [HL2737] used in Western Blot (WB). GTX639566
Anti-Insulin Receptor beta antibody [HL2737] used in Western Blot (WB). GTX639566
Anti-Insulin Receptor beta antibody [HL2737] used in IHC (Paraffin sections) (IHC-P). GTX639566

Cat. No. GTX639566

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2737

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Insulin Receptor beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Insulin Receptor beta

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Insulin Receptor beta antibody [HL2737] used in Western Blot (WB). GTX639566

GTX639566 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor Beta antibody [HL2737] (GTX639566) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Insulin Receptor beta antibody [HL2737] used in Western Blot (WB). GTX639566

GTX639566 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor Beta antibody [HL2737] (GTX639566) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Insulin Receptor beta antibody [HL2737] used in IHC (Paraffin sections) (IHC-P). GTX639566

GTX639566 IHC-P Image

Insulin Receptor beta antibody [HL2737] detects Insulin Receptor beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Insulin Receptor beta stained by Insulin Receptor beta antibody [HL2737] (GTX639566) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations