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Insulin Receptor beta antibody

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136
Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

Cat. No. GTX101136

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Positive Control

NIH-3T3 , PC-12 , rat liver

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Insulin Receptor beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101136

GTX101136 IHC-P Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Insulin Receptor beta antibody (GTX101136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX101136

GTX101136 IHC-P Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Insulin Receptor beta antibody (GTX101136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX101136 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Various whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin Receptor beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101136

GTX101136 ICC/IF Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein at cytoplasm and cell membrane by immunofluorescent analysis.
Sample: RT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Insulin Receptor stained by Insulin Receptor beta antibody (GTX101136) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor Beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor Beta antibody (GTX101136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin Receptor beta antibody used in Western Blot (WB). GTX101136

GTX101136 WB Image

Insulin Receptor beta antibody detects Insulin Receptor beta protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Insulin Receptor beta antibody (GTX101136) at a dilution of 1:1000.

REFERENCE

REVIEW

Insulin Receptor beta antibody Cat. No. GTX101136

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169