Insulin Receptor beta (phospho Tyr1322) antibody

Cat. No. GTX55015

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399)

See all Insulin Receptor products

Datasheet
Document

View product citations for antibody GTX55015 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Product Note

The full-length Insulin Receptor includes the 27 aa signal peptide that is cleaved during maturation. The Tyr1322 of mature Insulin Receptor (P06213-2) is equivalent to Tyr1349 of uncleaved Insulin Receptor or Tyr1361 of uncleaved Insulin Receptor (P06213-1)..

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Insulin Receptor (pY1322). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Gene ID: 3643 INSR
UniProt: P06213 INSR

Research Area

DATA IMAGES

GTX55015 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55015 Insulin Receptor beta (phospho Tyr1322) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX55015 WB Image

WB analysis of THP1 (A), HEK293T (B) whole cell lysates using GTX55015 Insulin Receptor beta (phospho Tyr1322) antibody.

GTX55015 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX55015 Insulin Receptor beta (phospho Tyr1322) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty