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Insulin Receptor (phospho Tyr960) antibody

Anti-Insulin Receptor (phospho Tyr960) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25678
Anti-Insulin Receptor (phospho Tyr960) antibody used in Western Blot (WB). GTX25678

Cat No. GTX25678

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Product Note

The full-length Insulin Receptor includes the 27 aa signal peptide that is cleaved during maturation. The Tyr960 of mature Insulin Receptor(P06213-2) is equivalent toTyr972 of mature Insulin Receptor (P06213-1).

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from the region of the human Insulin Receptor that contains tyrosine 960.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380646

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Insulin Receptor (phospho Tyr960) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25678

GTX25678 ICC/IF Image

ICC/IF analysis of MCF-7 cells with insulin treatment (100nM for 5 min) using GTX25678 Insulin Receptor (phospho Tyr960) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

Anti-Insulin Receptor (phospho Tyr960) antibody used in Western Blot (WB). GTX25678

GTX25678 WB Image

WB (peptide competition) analysis of CHO-T cells transfected with an insulin receptor-containing vector and stimulated with 50 nM insulin for 5 minutes (Lane 2-5) using GTX25678 Insulin Receptor (phospho Tyr960) antibody prior incubated with the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf