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Insulin antibody [GT1229]

Recombinant antibody
Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826
Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826
Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826
Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826
Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826
Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826
Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826
Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826
Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826

Cat No. GTX02826

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1229

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($369)
See all Insulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 0.05% BSA, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Insulin

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826

GTX02826 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826

GTX02826 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826

GTX02826 IHC-P Image

Insulin antibody [GT1229] detects Insulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Insulin stained by Insulin antibody [GT1229] (GTX02826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826

GTX02826 IHC-P Image

Insulin antibody [GT1229] detects Insulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Insulin stained by Insulin antibody [GT1229] (GTX02826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826

GTX02826 IHC-P Image

IHC-P analysis of mouse pancreas tissue section using Insulin antibody [GT1229] Insulin antibody [GT1229].
Dilution : 1:100
Blue : DAPI for nuclear staining.

Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826

GTX02826 IHC-P Image

IHC-P analysis of rat pancreas tissue (pancreatic islet) using GTX02826 Insulin antibody [GT1229].
Dilution : 1:100

Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826

GTX02826 IHC-P Image

IHC-P analysis of rat pancreas tissue section using Insulin antibody [GT1229] Insulin antibody [GT1229].
Dilution : 1:100
Blue : DAPI for nuclear staining.

Anti-Insulin antibody [GT1229] used in IHC (Paraffin sections) (IHC-P). GTX02826

GTX02826 IHC-P Image

IHC-P analysis of mouse pancreas tissue (pancreatic islet) using GTX02826 Insulin antibody [GT1229].
Dilution : 1:100

Anti-Insulin antibody [GT1229] used in Western Blot (WB). GTX02826

GTX02826 WB Image

WB analysis of various samples using GTX02826 Insulin antibody [GT1229].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 369