Insulin antibody [HL1393]

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:3000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Indirect ELISA analysis was performed by coating the plate with recombinant E. coli expressed, human insulin protein (86.21-1.35 nM). Coated protein was probed with Insulin antibody [HL1393] (GTX636833) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Insulin antibody [HL1393] detects Insulin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse pancreas.
Green: Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:200.
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas.
Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Recombinant human insulin were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.