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Insulin antibody [HL1393]

Recombinant antibody Orthogonal
Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833
Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833
Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833
Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833
Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833

Cat. No. GTX636833

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1393

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Insulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:5000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human insulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833

GTX636833 IHC-P Image

Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833

GTX636833 IHC-P Image

Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Insulin antibody [HL1393] used in IHC (Paraffin sections) (IHC-P). GTX636833

GTX636833 IHC-P Image

Insulin antibody [HL1393] detects secreted Insulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas.
Insulin stained by Insulin antibody [HL1393] (GTX636833) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833

GTX636833 WB Image

Recombinant human insulin were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Insulin antibody [HL1393] used in Western Blot (WB). GTX636833

GTX636833 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [HL1393] (GTX636833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations