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Insulin antibody

Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334
Anti-Insulin antibody used in Western Blot (WB). GTX64334
Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334
Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334
Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334

Cat. No. GTX64334

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399)
See all Insulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS (NP_000198.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334

GTX64334 IHC-P Image

IHC-P analysis of mouse pancreatic islet tissue using GTX64334 Insulin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Insulin antibody used in Western Blot (WB). GTX64334

GTX64334 WB Image

WB analysis of mouse pancreas tissue lysate using GTX64334 Insulin antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334

GTX64334 IHC-P Image

IHC-P analysis of mouse pancreatic islet tissue using GTX64334 Insulin antibody.
Dilution : 1:50

Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334

GTX64334 IHC-P Image

IHC-P analysis of rat pancreatic islet tissue using GTX64334 Insulin antibody.
Dilution : 1:50

Anti-Insulin antibody used in IHC (Paraffin sections) (IHC-P). GTX64334

GTX64334 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX64334 Insulin antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399