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Integrin alpha 2 antibody [16B4]

Anti-Integrin alpha 2 antibody [16B4] used in Flow cytometry (FACS). GTX75705

Cat No. GTX75705

Host

Mouse

Clonality

Monoclonal

Clone Name

16B4

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Functional Assay

Reactivity

Human
Package
200 μg ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 30-40μg/ml
FACS 30μg/ml
IP 25-50μg/ml
ELISA 10μg/ml
Functional Assay Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Product Note

This antibody recognizes human Integrin alpha 2.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376975

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit alpha 2 , BR , CD49B , GPIa , HPA-5 , VLA-2 , VLAA2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 2 antibody [16B4] used in Flow cytometry (FACS). GTX75705

GTX75705 FACS Image

FACS analysis of human peripheral blood platelets using GTX75705 Integrin alpha 2 antibody [16B4].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf