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Integrin alpha 2 antibody [16B4]

Anti-Integrin alpha 2 antibody [16B4] used in Flow cytometry (FACS). GTX75705

Cat No. GTX75705

Host

Mouse

Clonality

Monoclonal

Clone Name

16B4

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Functional Assay

Reactivity

Human
Package
200 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 30-40μg/ml
FACS 30μg/ml
IP 25-50μg/ml
ELISA 10μg/ml
Functional Assay Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes human Integrin alpha 2.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Integrin Subunit Alpha 2 , Br , Cd49B , Gpia , Hpa-5 , Vla-2 , Vlaa2 , Itga2

Background

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 2 antibody [16B4] used in Flow cytometry (FACS). GTX75705

GTX75705 FACS Image

FACS analysis of human peripheral blood platelets using GTX75705 Integrin alpha 2 antibody [16B4].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379