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Integrin alpha 2 antibody [GT1238]

Anti-Integrin alpha 2 antibody [GT1238] used in Western Blot (WB). GTX02835
Anti-Integrin alpha 2 antibody [GT1238] used in IHC (Paraffin sections) (IHC-P). GTX02835
Anti-Integrin alpha 2 antibody [GT1238] used in Western Blot (WB). GTX02835
Anti-Integrin alpha 2 antibody [GT1238] used in Western Blot (WB). GTX02835

Cat No. GTX02835

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1238

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Observed MW

150-160 kDa. The observed M.W. is based on the following publication. PMID: 19411307|18787945

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 0.05% BSA, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Integrin alpha 2

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit alpha 2 , BR , CD49B , GPIa , HPA-5 , VLA-2 , VLAA2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 2 antibody [GT1238] used in Western Blot (WB). GTX02835

GTX02835 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Integrin alpha 2 antibody [GT1238] (GTX02835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 19411307 and PMID: 18787945

Anti-Integrin alpha 2 antibody [GT1238] used in IHC (Paraffin sections) (IHC-P). GTX02835

GTX02835 IHC-P Image

IHC-P analysis of human colon carcinoma section using GTX02835 Integrin alpha 2 antibody [GT1238].
Dilution : 1:100

Anti-Integrin alpha 2 antibody [GT1238] used in Western Blot (WB). GTX02835

GTX02835 WB Image

WB analysis of various samples using GTX02835 Integrin alpha 2 antibody [GT1238].
Dilution : 1:1000
Loading : 25μg

Anti-Integrin alpha 2 antibody [GT1238] used in Western Blot (WB). GTX02835

GTX02835 WB Image

WB analysis of A549 whole cell lysate using GTX02835 Integrin alpha 2 antibody [GT1238].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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