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Integrin alpha 4 antibody [9F10] (APC)

Anti-Integrin alpha 4 antibody [9F10] (APC) used in Flow cytometry (FACS). GTX01494-07

Cat. No. GTX01494-07

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9F10

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($349)
See all Integrin alpha 4 products
PRODUCT

Summary

The 9F10 monoclonal antibody reacts with human CD49d, a 150kD integral membrane protein that is also known as integrin αalpha4 (ITGA4). Together with integrin αbeta1 (CD29), CD49d forms the VLA-4 heterodimer that serves as a receptor for fibronectin and VCAM-1. VLA-4 is widely expressed on lymphocytes, monocytes, thymocytes, and NK cells. CD49d can also dimerize with integrin αbeta7 to form a receptor that binds the mucosal vascular addressin molecule, MAdCAM-1. CD49d is involved in cellular adhesion to other cells as well as extracellular matrix.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 4 , CD49D , IA4 , ITGA4 , Integrin alpha 4 , CD49d

Cellular Localization

Membrane

Background

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 4 antibody [9F10] (APC) used in Flow cytometry (FACS). GTX01494-07

GTX01494-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01494-07 Integrin alpha 4 antibody [9F10] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

REFERENCE

Clone Reference

2018; ALT-803 Transiently Reduces Simian Immunodeficiency Virus Replication in the Absence of Antiretroviral Treatment.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 349