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Integrin alpha 4 antibody [HP2/1]

Cat. No. GTX76407

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HP2/1

Isotype

 IgG1

Application

 ICC/IF, IHC-Fr, FACS, IP, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Rabbit, Goat, Bovine, Cat, Pig, Cynomolgus monkey, Horse, Llama, Mink, Mustelid, Rhesus Monkey
Package
200 μg ($469)
See all Integrin alpha 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1μg/5 x 105 cells
IP Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Mouse anti Human CD49d, clone HP2/1 can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1 (Weller et al. 1991).
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

JM leukaemia line.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_377322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 4 , CD49D , IA4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

REFERENCE

Application Reference

Hernandez AA et al. J Immunol 2020; 205 (10) : 2806-2820 An Allosteric Shift in CD11c Affinity Activates a Proatherogenic State in Arrested Intermediate Monocytes.
Application : ICC/IF
Reactivity : Human
Abplanalp WT et al. Diabetes 2016; Enhanced Integrin α4β1-Mediated Adhesion Contributes to a Mobilization Defect of Endothelial Progenitor Cells in Diabetes.
Application : IP
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469