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Integrin alpha 4 antibody [R1-2] (PerCP-Cy5.5)

Anti-Integrin alpha 4 antibody [R1-2] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01513-11

Cat. No. GTX01513-11

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 R1-2

Isotype

 IgG2b

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($409)
See all Integrin alpha 4 products
PRODUCT

Summary

The R1-2 monoclonal antibody reacts with mouse CD49d, a 150kD glycoprotein member of the integrin αfamily. CD49d is also known as integrin αalpha4 (ITGA4). Together with integrin αbeta1 (CD29), CD49d forms the VLA-4 heterodimer that serves as a receptor for fibronectin and VCAM-1. VLA-4 is widely expressed on lymphocytes, monocytes, thymocytes, and NK cells. CD49d can dimerize with integrin αbeta7 to form a receptor that binds the mucosal vascular addressin molecule, MAdCAM-1.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 4 antibody [R1-2] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01513-11

GTX01513-11 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01513-11 Integrin alpha 4 antibody [R1-2] (PerCP-Cy5.5).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

REFERENCE

Clone Reference

Kitai Y et al. Int Immunol 2021; (Epub) CD47 promotes T-cell lymphoma metastasis by upregulating AKAP13-mediated RhoA activation.
2001; alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409