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Integrin alpha 4 antibody

Anti-Integrin alpha 4 antibody used in Western Blot (WB). GTX31781
Anti-Integrin alpha 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31781

Cat. No. GTX31781

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all Integrin alpha 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Rat Spleen Tissue Lysate(GTX27945)

Product Note

At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Integrin alpha 4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Integrin alpha 4.The immunogen is located within amino acids 220 - 270 of Integrin alpha 4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 4 , CD49D , IA4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 4 antibody used in Western Blot (WB). GTX31781

GTX31781 WB Image

WB analysis of rat spleen tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31781 Integrin alpha 4 antibody.
Working concentration : 1 μg/ml

Anti-Integrin alpha 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31781

GTX31781 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31781 Integrin alpha 4 antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419