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Integrin alpha 5 antibody [10F6]

Anti-Integrin alpha 5 antibody [10F6] used in Western Blot (WB). GTX83192
Anti-Integrin alpha 5 antibody [10F6] used in Flow cytometry (FACS). GTX83192
Anti-Integrin alpha 5 antibody [10F6] used in IHC (Paraffin sections) (IHC-P). GTX83192

Cat. No. GTX83192

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10F6

Isotype

 IgG2a

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Integrin alpha 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ITGA5 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 5 , CD49e , FNRA , VLA-5 , VLA5A

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 5 antibody [10F6] used in Western Blot (WB). GTX83192

GTX83192 WB Image

WB analysis of ITGA5-hIgGFc transfected HEK293 cell lysate using GTX83192 Integrin alpha 5 antibody [10F6].

Anti-Integrin alpha 5 antibody [10F6] used in Flow cytometry (FACS). GTX83192

GTX83192 FACS Image

FACS analysis of HeLa cells using GTX83192 Integrin alpha 5 antibody [10F6].
Green : Integrin alpha 5
Purple : negative control

Anti-Integrin alpha 5 antibody [10F6] used in IHC (Paraffin sections) (IHC-P). GTX83192

GTX83192 IHC-P Image

IHC-P analysis of human Placenta tissue using GTX83192 Integrin alpha 5 antibody [10F6].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399