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Integrin alpha 5 antibody

Orthogonal
Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705
Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705
Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705
Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705

Cat. No. GTX130705

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Integrin alpha 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Positive Control

MDA-MB-231 membrane fraction extract , mouse liver , rat liver , U87-MG , SK-N-SH , SK-N-AS

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Integrin alpha 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886325

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 5 , CD49e , FNRA , VLA-5 , VLA5A

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705

GTX130705 WB Image

Orthogonal

Integrin alpha 5 antibody detects Integrin alpha 5 protein by western blot analysis. Whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 5 antibody (GTX130705) diluted at 1:5000.

Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705

GTX130705 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Integrin alpha 5 antibody (GTX130705) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705

GTX130705 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Integrin alpha 5 antibody (GTX130705) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX130705

GTX130705 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 5 antibody (GTX130705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Terasaki M et al. Nutr Cancer 2021; : 1-16 A Marine Carotenoid of Fucoxanthinol Accelerates the Growth of Human Pancreatic Cancer PANC-1 Cells.
Terasaki M et al. Cancer Genomics Proteomics 2021; 18 (2) : 133-146 A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell.
Application : WB
Reactivity : Mouse
Inaba H et al. PLoS One 2019; 14 (3) : e0213309 Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae.
Application : WB
Reactivity : Human
Wu CC et al. Biomaterials 2018; 185 : 142-154 Synthetic α5β1 integrin ligand PHSRN is proangiogenic and neuroprotective in cerebral ischemic stroke.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169