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Integrin alpha 5 antibody

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249
Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249
Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249
Anti-Integrin alpha 5 antibody used in Flow cytometry (FACS). GTX35249
Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249
Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249
Anti-Integrin alpha 5 antibody used in Flow cytometry (FACS). GTX35249
Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249
Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX35249
Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

Cat. No. GTX35249

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all Integrin alpha 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

114.54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Membrane

Background

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of rat cardiac muscle tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of human placenta tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Integrin alpha 5 antibody used in Flow cytometry (FACS). GTX35249

GTX35249 FACS Image

FACS analysis of A431 cells using GTX35249 Integrin alpha 5 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of mouse cardiac muscle tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of human colon cancer tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Integrin alpha 5 antibody used in Flow cytometry (FACS). GTX35249

GTX35249 FACS Image

FACS analysis of HT-1080 cells using GTX35249 Integrin alpha 5 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of human placenta tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Integrin alpha 5 antibody used in Western Blot (WB). GTX35249

GTX35249 WB Image

WB analysis of various samples using GTX35249 Integrin alpha 5 antibody.
Lane 1 : human HeLa cell lysate
Lane 2 : human HepG2 cell lysate
Lane 3 : rat liver tissue lysate
Lane 4 : mouse liver tissue lysate
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-Integrin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35249

GTX35249 IHC-P Image

IHC-P analysis of human colon cancer tissue using GTX35249 Integrin alpha 5 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 429