GeneTex
United States (US)

Integrin alpha 5 (cleaved Phe42) antibody

ITGA5-cleaved-Phe42-antibody-GTX86905-WB-1_18121411_200.jpg

Cat No. GTX86905

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

92 kDa. ( Note )

Specificity/Sensitivity

ITGA5 (heavy chain,Cleaved-Phe42) Antibody detects endogenous levels of fragment of activated ITGA5 resulting from cleavage adjacent to Phe42.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ITGA5.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Alpha 5,Cd49E,Fnra,Vla-5,Vla5A,Itga5

Background

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Database

Research Area

DATA IMAGES
ITGA5-cleaved-Phe42-antibody-GTX86905-WB-1_18121411_200.jpg

GTX86905 WB Image

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 24h, using ITGA5 (heavy chain,Cleaved-Phe42) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 349