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Integrin alpha 5 (cleaved Phe42) antibody

Anti-Integrin alpha 5 (cleaved Phe42) antibody used in Western Blot (WB). GTX86905

Cat. No. GTX86905

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μg ($399)
See all Integrin alpha 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Product Note

Integrin alpha 5 (cleaved Phe42) antibody detects endogenous levels of fragment of activated ITGA5 resulting from cleavage adjacent to Phe42.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Integrin alpha 5 (23-72).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10723501

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD49e , FNRA , ITGA5 , VLA5 , VLA5A , integrin subunit alpha 5 , Integrin alpha 5 , Integrin α5 , Integrin αV , Integrin alpha V

Cellular Localization

Membrane

Background

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 5 (cleaved Phe42) antibody used in Western Blot (WB). GTX86905

GTX86905 WB Image

WB analysis of NIH/3T3 cells treated with etoposide 25uM (24h) lysate using GTX86905 Integrin alpha 5 (cleaved Phe42) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Juli?n Albarr?n-Ju?rez et al. J Exp Med 2018; 215 (10) : 2655-2672 Piezo1 and G q/G 11 Promote Endothelial Inflammation Depending on Flow Pattern and Integrin Activation.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399