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Integrin alpha 6 antibody [450-30A]

Anti-Integrin alpha 6 antibody [450-30A] used in Flow cytometry (FACS). GTX75046
Anti-Integrin alpha 6 antibody [450-30A] used in Flow cytometry (FACS). GTX75046
Anti-Integrin alpha 6 antibody [450-30A] used in Western Blot (WB). GTX75046

Cat No. GTX75046

Host

Mouse

Clonality

Monoclonal

Clone Name

450-30A

Isotype

IgG1

Application

WB, IHC-Fr, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-Fr 1/50-1/100
FACS 1/10-1/50

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Recommend the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

integrin a6b4 purified from A431 cells

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Integrin Subunit Alpha 6 , Cd49F , Itga6B , Vla-6 , Itga6

Background

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 6 antibody [450-30A] used in Flow cytometry (FACS). GTX75046

GTX75046 FACS Image

FACS analysis of human peripheral blood monocytes using GTX75046 Integrin alpha 6 antibody [450-30A].

Anti-Integrin alpha 6 antibody [450-30A] used in Flow cytometry (FACS). GTX75046

GTX75046 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX75046 Integrin alpha 6 antibody [450-30A].

Anti-Integrin alpha 6 antibody [450-30A] used in Western Blot (WB). GTX75046

GTX75046 WB Image

WB analysis of Jurkat cell lysate using GTX75046 Integrin alpha 6 antibody [450-30A].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf