GeneTex
United States (US)

Integrin alpha 6 antibody [N3C2], Internal

KO/KD
GTX100565_39869_WB_hESC_19011506_548.jpg
GTX100565_39869_20151119_WB_M_lung_19011506_205.jpg
GTX100565_39869_20151105_WB_R_lung_19011506_563.jpg

Cat No. GTX100565

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

Human ESC , Rat lung

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Integrin alpha 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD49f antibody, DKFZp686J01244 antibody, FLJ18737 antibody, ITGA6B antibody, VLA-6 antibody, ITGA6 antibody, CD49 antigen-like family member F antibody, integrin alpha-6 antibody, integrin alpha6B antibody, "integrin, alpha 6 antibody"

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100565_39869_WB_hESC_19011506_548.jpg

GTX100565 WB Image

Sample (20 µg of whole cell lysate)
A: human ESC
5% SDS PAGE
GTX100565 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100565_39869_20151119_WB_M_lung_19011506_205.jpg

GTX100565 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 6 antibody [N3C2], Internal (GTX100565) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100565_39869_20151105_WB_R_lung_19011506_563.jpg

GTX100565 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 6 antibody [N3C2], Internal (GTX100565) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Integrin alpha 6 antibody [N3C2], Internal Cat No. GTX100565

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 3 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 80 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, overnightHr
Lane Description : Lane 1: OC3 with siCtrl, Lane 2: OC3 with siXXXX, Lane 3: OC3-IV2 with siCtrl, Lane 4: OC3-IV2 with siXXXX
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 2Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: MDA-MB-231 cells, Lane 2: knockdown of human Integrin alpha 6 in MDA-MB-231 cells
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% milk, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169