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Integrin alpha 6 antibody [N3C2], Internal

KO/KD
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

Cat. No. GTX100565

Reference 3
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Integrin alpha 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

Human ESC , rat lung , mouse lung

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Integrin alpha 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950600

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 6 , CD49f , ITGA6B , VLA-6

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 6 antibody [N3C2], Internal (GTX100565) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 6 antibody [N3C2], Internal (GTX100565) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

Sample (20 μg of whole cell lysate)
A: human ESC
5% SDS PAGE
GTX100565 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565

GTX100565 WB Image

KO/KD

The data was published in the journal Elife in 2019.PMID: 30801249

REFERENCE

Application Reference

Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Rivera-Serrano EE et al. Elife 2019; 8 Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses.
Sproule TJ et al. PLoS Genet 2014; 10 (2) : e1004068 Molecular identification of collagen 17a1 as a major genetic modifier of laminin gamma 2 mutation-induced junctional epidermolysis bullosa in mice.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

Integrin alpha 6 antibody [N3C2], Internal Cat. No. GTX100565

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 80 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, 16Hr
Lane Description : Lane 1: OC3 with siCtrl, Lane 2: OC3 with siXXXX, Lane 3: OC3-IV2 with siCtrl, Lane 4: OC3-IV2 with siXXXX
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 2Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: MDA-MB-231 cells, Lane 2: knockdown of human Integrin alpha 6 in MDA-MB-231 cells
Anti-Integrin alpha 6 antibody [N3C2], Internal used in Western Blot (WB). GTX100565
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% milk, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169