GeneTex
United States (US)

Integrin alpha V antibody [C2C3], C-term

KO/KD
GTX100789_39911_CT_WB_19011506_839.jpg
GTX100789_39911_WB_19011506_710.jpg
GTX100789_39911_IHC_19011506_769.jpg

Cat No. GTX100789

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

RD (Rhabdomyosarcoma) cell (Mock control) , RD transfected scramble siRNA , HUVEC

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Integrin alpha V. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD51 antibody, DKFZp686A08142 antibody, MSK8 antibody, VNRA antibody, ITGAV antibody, integrin alpha-V antibody, antigen identified by monoclonal antibody L230 antibody, vitronectin receptor subunit alpha antibody, "integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody"

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100789_39911_CT_WB_19011506_839.jpg

GTX100789 WB Image

KO/KD

Sample (30 µg of whole cell lysate)
A: RD (Rhabdomyosarcoma) cell (Mock control)
B: RD transfected scramble siRNA
C: RD transfected ITGAV siRNA
8% SDS PAGE
GTX100789 diluted at 1:900
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100789_39911_WB_19011506_710.jpg

GTX100789 WB Image

Sample (30 µg of whole cell lysate)
A: HUVEC
7.5% SDS PAGE
GTX100789 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100789_39911_IHC_19011506_769.jpg

GTX100789 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin alpha V(GTX100789) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169