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Integrin alpha V antibody [C2C3], C-term

KO/KD Orthogonal
Anti-Integrin alpha V antibody [C2C3], C-term used in Western Blot (WB). GTX100789
Anti-Integrin alpha V antibody [C2C3], C-term used in Western Blot (WB). GTX100789
Anti-Integrin alpha V antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100789
Anti-Integrin alpha V antibody [C2C3], C-term used in Western Blot (WB). GTX100789

Cat. No. GTX100789

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Integrin alpha V products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

RD , HUVEC , A549 membrane extracts

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Integrin alpha V. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha V , CD51 , MSK8 , VNRA , VTNR

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Integrin alpha V antibody [C2C3], C-term used in Western Blot (WB). GTX100789

GTX100789 WB Image

KO/KD

Sample (30 μg of whole cell lysate)
A: RD (Rhabdomyosarcoma) cell (Mock control)
B: RD transfected scramble siRNA
C: RD transfected ITGAV siRNA
8% SDS PAGE
GTX100789 diluted at 1:900
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha V antibody [C2C3], C-term used in Western Blot (WB). GTX100789

GTX100789 WB Image

Sample (30 μg of whole cell lysate)
A: HUVEC
7.5% SDS PAGE
GTX100789 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin alpha V antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100789

GTX100789 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin alpha V(GTX100789) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Integrin alpha V antibody [C2C3], C-term used in Western Blot (WB). GTX100789

GTX100789 WB Image

Orthogonal

A549 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha V antibody [C2C3], C-term (GTX100789) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tang WF et al. J Proteome Res 2018; (Epub) Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71.
Application : WB
Reactivity : Human
Hung CJ et al. J Endod 2015; 40 (11) : 1802-9 The Role of Integrin av in Proliferation and Differentiation of Human Dental Pulp Cell Response to Calcium Silicate Cement
Application : WB
Reactivity : Human
Zhang J et al. PLoS One 2014; 9 (3) : e92252 A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling.
Application : WB
Reactivity : Human
Suzuki S et al. PLoS One 2014; 9 (11) : e112490 Adhesive and migratory effects of phosphophoryn are modulated by flanking peptides of the integrin binding motif.
Application : ELISA
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production