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Integrin beta 1 / CD29 antibody [12G10]

Anti-Integrin beta 1 / CD29 antibody [12G10] used in Flow cytometry (FACS). GTX75707

Cat. No. GTX75707

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12G10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Mink
Package
200 μg ($459)
See all Integrin beta 1 / CD29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/25-1/50
IP Assay dependent
ELISA 10μg/ml

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane , Cell junction

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody [12G10] used in Flow cytometry (FACS). GTX75707

GTX75707 FACS Image

FACS analysis of human peripheral blood monocytes using GTX75707 Integrin beta 1 / CD29 antibody [12G10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459