APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
FACS
Cells were fixed in 4% paraformaldehyde before staining.
Neutralizing/Inhibition
Antibody BV7 functions as an inhibiting antibody. The antibody was functionally tested by adhesion assay, chemotaxis, patch clamp analysis and neurotoxicity induced apoptosis.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody stains the extracellular domain of beta-1 integrin. The monoclonal antibody BV7 does not recognize α5β1 complex and not the cytoplasmic part of theβ1-subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human umbilical vein EC
Purification
Purified IgG1
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD29,FNRB,GPIIA,ITGB1,MDF2,MSK12,VLAB,VLABETA,integrin subunit beta 1,Integrin beta 1,B1 Integrin,β1 integrin
Cellular Localization
Cell membrane,Cell projection,Invadopodium membrane,Ruffle membrane,Recycling endosome,Melanosome,Cleavage furrow,Lamellipodium,Ruffle,Sarcolemma,Cell junction
Background
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for Integrin beta 1 / CD29 antibody [BV7] (GTX54427). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Integrin beta 1 / CD29 antibody [BV7] (GTX54427). Be the first to share your experience with this product.