PRODUCT
Summary
Integrin beta-1 / CD29 antibody detects integrin beta-1 protein (ITGB1), also known as CD29, which forms heterodimeric receptors with at least ten integrin alpha proteins to mediate various aspects of cell adhesion and migration. These functions are essential for embryogenesis, tissue repair, immune function, and other crucial processes in multicellular organisms. Integrin beta-1 has a predicted molecular weight of 88 kDa and exists in multiple isoforms.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910031
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
Cellular Localization
Cell membrane,Cell junction
Background
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX636657 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636657 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636657 WB Image
Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636657 IHC-P Image
Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissues.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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