GeneTex
  • Country / Location Selection

United States (US)

Integrin beta 1 / CD29 antibody [HL1255]

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657
Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657
Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657
Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657
Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657
Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657
Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

Cat No. GTX636657

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1255

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)
PRODUCT

Summary

Integrin beta-1 / CD29 antibody detects integrin beta-1 protein (ITGB1), also known as CD29, which forms heterodimeric receptors with at least ten integrin alpha proteins to mediate various aspects of cell adhesion and migration. These functions are essential for embryogenesis, tissue repair, immune function, and other crucial processes in multicellular organisms. Integrin beta-1 has a predicted molecular weight of 88 kDa and exists in multiple isoforms.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910031

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cell junction

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

GTX636657 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

GTX636657 WB Image

Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657

GTX636657 IHC-P Image

Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657

GTX636657 IHC-P Image

Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

GTX636657 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

GTX636657 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

GTX636657 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for Integrin beta 1 / CD29 antibody [HL1255] (GTX636657). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Integrin beta 1 / CD29 antibody [HL1255] (GTX636657). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations