Integrin beta 1 / CD29 antibody [HL1255]

Name: Integrin beta 1 / CD29 antibody [HL1255]
Brand: GeneTex
SKU: GTX636657
Price: 399.00 USD

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in Western Blot (WB). GTX636657

Anti-Integrin beta 1 / CD29 antibody [HL1255] used in IHC (Paraffin sections) (IHC-P). GTX636657

Cat. No. GTX636657

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1255
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all Integrin beta 1 / CD29 products

Datasheet
Reference

PRODUCT

Summary

Integrin beta-1 / CD29 antibody detects integrin beta-1 protein (ITGB1), also known as CD29, which forms heterodimeric receptors with at least ten integrin alpha proteins to mediate various aspects of cell adhesion and migration. These functions are essential for embryogenesis, tissue repair, immune function, and other crucial processes in multicellular organisms. Integrin beta-1 has a predicted molecular weight of 88 kDa and exists in multiple isoforms.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910031

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cell junction

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3688 ITGB1
UniProt: P05556 ITGB1

Research Area

DATA IMAGES

GTX636657 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636657 IHC-P Image

Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636657 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636657 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX636657 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX636657 WB Image

Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636657 IHC-P Image

Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636657 IHC-P Image

Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636657 IHC-P Image

Integrin beta 1 / CD29 antibody [HL1255] detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog tissues.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1255] (GTX636657) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tan X et al. J Clin Invest 2023; 133 (7) EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer.

Application : WB, ICC/IF

Reactivity : Human

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REVIEW

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Application Reference

Tan X et al. J Clin Invest 2023; 133 (7) EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer.

Application : WB, ICC/IF

Reactivity : Human

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

Integrin beta 1 / CD29 antibody [HL1255]

Cat. No. GTX636657

Host

Clonality

Clone Name

Isotype

Application

Reactivity

Summary

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX636657 WB Image

GTX636657 IHC-P Image

GTX636657 WB Image

GTX636657 WB Image

GTX636657 WB Image

GTX636657 WB Image

GTX636657 WB Image

GTX636657 IHC-P Image

GTX636657 IHC-P Image

GTX636657 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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