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Integrin beta 1 / CD29 antibody [HL1256]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

Cat. No. GTX636658

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1256

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Integrin beta 1 / CD29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910032

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cell junction

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658

GTX636658 ICC/IF Image

Integrin beta 1 / CD29 antibody [HL1256] detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

KO/KD

Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations