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Integrin beta 1 / CD29 antibody [HL1256]

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658
Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

Cat. No. GTX636658

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1256

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Product Note

This antibody was raised against human Integrin beta 1 / CD29 Intracellular domain.

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910032

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cell junction

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (100 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636658

GTX636658 ICC/IF Image

Integrin beta 1 / CD29 antibody [HL1256] detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Wild-type (WT) and ITGB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody [HL1256] used in Western Blot (WB). GTX636658

GTX636658 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody [HL1256] (GTX636658) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations