GeneTex
  • Country / Location Selection

United States (US)

Integrin beta 1 / CD29 antibody

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971
Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112971
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX112971
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX112971

Cat No. GTX112971

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , human ITGB1-transfected 293T

Predict Reactivity

Rat, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722978

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Melanosome

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971

GTX112971 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112971

GTX112971 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971

GTX112971 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX112971) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971

GTX112971 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX112971

GTX112971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971

GTX112971 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX112971

GTX112971 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

There are currently no reviews for Integrin beta 1 / CD29 antibody (GTX112971). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf