GeneTex
United States (US)

Integrin beta 1 / CD29 antibody

KO/KD Overexpression
GTX112971_42753_20170216_WB_18121410_155.jpg
GTX112971_40408_IFA_18121410_342.jpg
GTX112971_40408_WB_B_18121410_825.jpg
GTX112971_40408_IHC_18121410_789.jpg
GTX112971_42753_20181026_WB_shRNA_watermark_19011519_300.jpg

Cat No. GTX112971

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , human ITGB1-transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD29 antibody, FNRB antibody, GPIIA antibody, MDF2 antibody, MSK12 antibody, VLA-BETA antibody, VLAB antibody, ITGB1 antibody, very late activation protein, beta polypeptide antibody, integrin beta-1 antibody, integrin VLA-4 beta subunit antibody, integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Melanosome

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112971_42753_20170216_WB_18121410_155.jpg

GTX112971 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112971_40408_IFA_18121410_342.jpg

GTX112971 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.

GTX112971_40408_WB_B_18121410_825.jpg

GTX112971 WB Image

Overexpression

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX112971) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112971_40408_IHC_18121410_789.jpg

GTX112971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112971_42753_20181026_WB_shRNA_watermark_19011519_300.jpg

GTX112971 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169