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Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody

Orthogonal
Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25189
Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody used in Western Blot (WB). GTX25189
Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody used in Western Blot (WB). GTX25189

Cat. No. GTX25189

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Cat
Package
50 μl ($399)
See all Integrin beta 1 / CD29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250
ICC/IF 1:250
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human integrin b1 receptor that contains threonines 788 and 789 (based on Swiss Protein database, accession number P05558). The sequence is conserved in human, mouse, rat and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380480

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cell junction

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25189

GTX25189 ICC/IF Image

Orthogonal

ICC/IF analysis of THP-1 cells treated with 100ng/mL of IFN-gamma for 15 minutes using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody used in Western Blot (WB). GTX25189

GTX25189 WB Image

Orthogonal

WB analysis of serum-starved mitotic HeLa cells generated by treatment with 100 ng/mL taxol for 16 hours using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody used in Western Blot (WB). GTX25189

GTX25189 WB Image

Orthogonal

WB analysis of membrane enriched extracts (30 μg lysate) of HeLa (Lane 1) treated with Paclitaxel (Lane 2), HepG2 (Lane 3) treated with Paclitaxel (Lane 4), and 10 μL of conditioned media from HepG2 cell line (Lane 5) using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody.
Dilution : 1:250

REFERENCE

Application Reference

Qin N et al. Eur J Med Chem 2016; 124 : 490-499 Synthesis and anti-metastatic effects of pregn-17(20)-en-3-amine derivatives.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399