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Integrin beta 3 antibody [GT1226]

Recombinant antibody Orthogonal
Anti-Integrin beta 3 antibody [GT1226] used in Western Blot (WB). GTX02823
Anti-Integrin beta 3 antibody [GT1226] used in IHC (Paraffin sections) (IHC-P). GTX02823
Anti-Integrin beta 3 antibody [GT1226] used in Western Blot (WB). GTX02823
Anti-Integrin beta 3 antibody [GT1226] used in IHC (Paraffin sections) (IHC-P). GTX02823

Cat. No. GTX02823

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1226

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all Integrin beta 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Observed MW

110 kDa. The observed M.W. is based on the following publication. PMID: 21426865

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Integrin beta 3

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 3 , BDPLT16 , BDPLT2 , CD61 , GP3A , GPIIIa , GT

Cellular Localization

Cell membrane

Background

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 3 antibody [GT1226] used in Western Blot (WB). GTX02823

GTX02823 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 3 antibody [GT1226] (GTX02823) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 21426865

Anti-Integrin beta 3 antibody [GT1226] used in IHC (Paraffin sections) (IHC-P). GTX02823

GTX02823 IHC-P Image

IHC-P analysis of rat bone marrow section using GTX02823 Integrin beta 3 antibody [GT1226].
Dilution : 1:100

Anti-Integrin beta 3 antibody [GT1226] used in Western Blot (WB). GTX02823

GTX02823 WB Image

WB analysis of various samples using GTX02823 Integrin beta 3 antibody [GT1226].
Dilution : 1:1000
Loading : 25μg

Anti-Integrin beta 3 antibody [GT1226] used in IHC (Paraffin sections) (IHC-P). GTX02823

GTX02823 IHC-P Image

IHC-P analysis of mouse bone marrow section using GTX02823 Integrin beta 3 antibody [GT1226].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 419