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Integrin beta 3 antibody [Y2/51]

Anti-Integrin beta 3 antibody [Y2/51] used in IHC (Paraffin sections) (IHC-P). GTX34801

Cat No. GTX34801

Host

Mouse

Clonality

Monoclonal

Clone Name

Y2/51

Isotype

IgG1

Application

ICC/IF, IHC-P, IHC-Fr

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT
IHC-Fr 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Specificity/Sensitivity

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the αV-chain (CD51) to form vitronectin receptor, or with the αIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

Phytohemagglutinin (PHA)-stimulated peripheral blood cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Integrin Subunit Beta 3 , Bdplt16 , Bdplt2 , Cd61 , Gp3A , Gpiiia , Gt , Itgb3

Background

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 3 antibody [Y2/51] used in IHC (Paraffin sections) (IHC-P). GTX34801

GTX34801 IHC-P Image

Formalin-fixed, paraffin-embedded human Spleen stained with CD61 Monoclonal Antibody (Y2/51).

REFERENCE

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REVIEW

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