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Integrin beta 4 antibody [450-9D] (Azide free)

Anti-Integrin beta 4 antibody [450-9D] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX75043

Cat. No. GTX75043

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 450-9D

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, IP

Reactivity

 Human, Ferret, Mustelid
Package
500 μg ($489)
See all Integrin beta 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr 1/500-1/1,000
FACS 1/25-1/100
IP Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Recommend the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

202 kDa. ( Note )

Product Note

This antibody recognizes an extracellular epitope on Integrin beta 4.

PROPERTIES

Form

Liquid

Buffer

Normal saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified alpha6 beta4 integrin from A431 cells.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376581

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 4 , CD104 , GP150

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 4 antibody [450-9D] (Azide free) used in IHC (Frozen sections) (IHC-Fr). GTX75043

GTX75043 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX75043 Integrin beta 4 antibody [450-9D] (Azide free).

REFERENCE

Application Reference

Drake JM et al. J Biol Chem 2010; 285 (44) : 33940-8 ZEB1 coordinately regulates laminin-332 and {beta}4 integrin expression altering the invasive phenotype of prostate cancer cells.
Application : FACS
Reactivity : Human
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Package List Price ($)
$ 489
Custom Conjugations