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Interferon gamma antibody [2G1]

Orthogonal
Anti-Interferon gamma antibody [2G1] used in ELISA (ELISA). GTX15624
Anti-Interferon gamma antibody [2G1] used in ELISA (ELISA). GTX15624

Cat. No. GTX15624

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G1

Isotype

 IgG1

Application

 WB, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
250 μg ($399)
See all Interferon gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX15624, Detection : GTX15751
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IFN gamma

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_2887520

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon gamma , IFG , IFI

Cellular Localization

Secreted

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Interferon gamma antibody [2G1] used in ELISA (ELISA). GTX15624

GTX15624 ELISA Image

Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 μg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 μg/ml.

Anti-Interferon gamma antibody [2G1] used in ELISA (ELISA). GTX15624

GTX15624 ELISA Image

Orthogonal

Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.

REFERENCE

Application Reference

Su Y et al. Clin Exp Immunol 2017; (Epub) Withasteroid B from D. metel L. Regulates Immune Responses by Modulating the JAK/STAT Pathway and the IL-17+ RORγt+ /IL-10+ FoxP3+ Ratio.
Application : WB
Reactivity : Mouse
Fang S et al. Neuromolecular Med 2016; Vorinostat Modulates the Imbalance of T Cell Subsets, Suppresses Macrophage Activity, and Ameliorates Experimental Autoimmune Uveoretinitis.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations