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Interferon gamma antibody [B133.5] (Biotin)

Orthogonal
Anti-Interferon gamma antibody [B133.5] (Biotin) used in ELISA (ELISA). GTX15751
Anti-Interferon gamma antibody [B133.5] (Biotin) used in ELISA (ELISA). GTX15751

Cat. No. GTX15751

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B133.5

Isotype

 IgG1

Application

 WB, IHC-P, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

 Human, Mouse
Package
250 μg ($399)
See all Interferon gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX15624, Detection : GTX15751
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 4% BSA

Preservative

Proprietary preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IFN gamma

Purification

Protein G purified

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon gamma , IFG , IFI

Cellular Localization

Secreted

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Interferon gamma antibody [B133.5] (Biotin) used in ELISA (ELISA). GTX15751

GTX15751 ELISA Image

Orthogonal

Purified human CD4+ T cells were left non-stimulated (bar 1), stimulated with anti-CD3 (bar 2), or stimulated with anti-CD3 and anti-CD28 (bar 3) for three days. Sandwich ELISA analysis of IFN gamma production using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 1:1000 and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 1:1000.

Anti-Interferon gamma antibody [B133.5] (Biotin) used in ELISA (ELISA). GTX15751

GTX15751 ELISA Image

Sandwich ELISA analysis of serial diluted human Interferon Gamma recombinant protein (1000, 400, 160, 64, 25.6, and 0 pg/ml, 50 ul per well) using GTX15624 Interferon gamma antibody [2G1] as coating antibody at 3 μg/ml and GTX15751 IFN gamma antibody [B133.5] (Biotin) as detecting antibody at 0.05 μg/ml.

REFERENCE

Clone Reference

M Kimoto et al. Nucleic Acids Res 2019; 47 (16):8362-8374 Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA
Application : Sandwich ELISA
U Yasamut et al. Front Immunol 2019; 10:1905 Neutralizing Activity of Anti-interferon-γ Autoantibodies in Adult-Onset Immunodeficiency Is Associated With Their Binding Domains
Application : Neutralizing/Inhibition
RE Sierra-Puente et al. Parasite Immunol 2009; 31 (7):384-91 Expression of immune modulator cytokines in human fulminant amoebic colitis
Application : IHC-P
CV Hartig et al. J Immunol 2000; 164 (5):2790-6 Naturally Developing Memory T Cell Xenoreactivity to Swine Antigens in Human Peripheral Blood Lymphocytes
Application : ELISA
Reactivity : Human, Mouse
JE Vaquerano et al. Biotechniques 1998; 25 (5):830-4 Digital quantification of the enzyme-linked immunospot (ELISPOT)
Application : ELISA
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399