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Interferon gamma antibody [R4-6A2] (Biotin)

Anti-Interferon gamma antibody [R4-6A2] (Biotin) used in ELISA (ELISA). GTX02943-02

Cat. No. GTX02943-02

Host

 Rat

Clonality

 Monoclonal

Clone Name

 R4-6A2

Isotype

 IgG1

Application

 ELISA, Sandwich ELISA

Reactivity

 Mouse
Package
250 μg ($399)
See all Interferon gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02927, Detection: GTX02943-02.
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant mouse IFN-γ.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Partially purified mouse IFN-γ

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Interferon gamma antibody [R4-6A2] (Biotin) used in ELISA (ELISA). GTX02943-02

GTX02943-02 ELISA Image

Sandwich ELISA analysis of mouse IFN-gamma protein using GTX02927 Interferon gamma antibody [AN18] as coating antibody and GTX02943-02 Interferon gamma antibody [R4-6A2] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 399