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Interferon gamma antibody [XMG1.2] (FITC)

Orthogonal
Anti-Interferon gamma antibody [XMG1.2] (FITC) used in Flow cytometry (FACS). GTX75119

Cat. No. GTX75119

Host

 Rat

Clonality

 Monoclonal

Clone Name

 XMG1.2

Isotype

 IgG1

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($369)
See all Interferon gamma products
PRODUCT

Summary

The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse IFN gamma.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_378381

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon gamma , IFN-g , Ifg

Cellular Localization

Secreted

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Interferon gamma antibody [XMG1.2] (FITC) used in Flow cytometry (FACS). GTX75119

GTX75119 FACS Image

Orthogonal

FACS analysis of C57Bl/6 splenocytes treated with PMA and Ionomycin using GTX75119 Interferon gamma antibody [XMG1.2] (FITC), followed by intracellular staining with FITC anti-mouse IFN gamma antibody.
Left panel : unstimulated
Right panel : PMA and Ionomycin
Antibody amount : 0.125 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369