ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 1 - 2 ?g/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For staining formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, for 10 min at 37?C. Note: Enzyme digestion is better than citrate buffer based epitope unmasking. IP: Use 2 ?g/mg protein lysate (Denatured verified; Use Protein G). WB: Use at a concentration of 1 - 2 ?g/ml. (Human form at 170 kDa; see under """"Specificity"""" for other species molecular weights.) Optimal dilutions/concentrations should be determined by the end user.
LS174T cells and skin
Recognizes involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine and a doublet of 105kD in porcine.
Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, 10mM PBS. pH 7.4
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Full length native protein (purified) (Human).
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.