APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2 μg/ml |
Note :
IHC-P
Digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC.
|
Not tested in other applications.
Calculated MW
Product Note
In Western blotting of cultured human keratinocytes, this antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig.
We do not recommend use of this product for Mouse samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length Involucrin protein (IVRN). An epitope maps between codon 421-568 of human involucrin.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
involucrin
Cellular Localization
Cytoplasm
Background
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX02654 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02654 Involucrin antibody [IVRN/2113R].
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REFERENCE
There are currently no references for Involucrin antibody [IVRN/2113R] (GTX02654). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Involucrin antibody [IVRN/2113R] (GTX02654). Be the first to share your experience with this product.