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Involucrin antibody [IVRN/2113R]

Anti-Involucrin antibody [IVRN/2113R] used in IHC (Paraffin sections) (IHC-P). GTX02654

Cat. No. GTX02654

Host

Rabbit

Clonality

Monoclonal

Clone Name

IVRN/2113R

Isotype

IgG

Application

IHC-P

Reactivity

Human, Dog, Pig, Monkey, Gorilla
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2 μg/ml

Note :

IHC-P
Digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC.

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

In Western blotting of cultured human keratinocytes, this antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig.
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length Involucrin protein (IVRN). An epitope maps between codon 421-568 of human involucrin.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

involucrin

Cellular Localization

Cytoplasm

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody [IVRN/2113R] used in IHC (Paraffin sections) (IHC-P). GTX02654

GTX02654 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02654 Involucrin antibody [IVRN/2113R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529