*Optimal dilutions/concentrations should be determined by the researcher.
Digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC.
Not tested in other applications.
In Western blotting of cultured human keratinocytes, this antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig.
We do not recommend use of this product for Mouse samples.
10mM PBS, 0.05% BSA
0.05% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
200 μg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human full-length Involucrin protein (IVRN). An epitope maps between codon 421-568 of human involucrin.
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
GTX02654 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02654 Involucrin antibody [IVRN/2113R].