GeneTex
  • Country / Location Selection

United States (US)

Involucrin antibody [IVRN/827]

Anti-Involucrin antibody [IVRN/827] used in IHC (Paraffin sections) (IHC-P). GTX34803

Cat No. GTX34803

Host

Mouse

Clonality

Monoclonal

Clone Name

IVRN/827

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.1-0.2ug/ml for 30 minutes at RT

Note :

IHC-P
Digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Purified involucrin from human keratinocytes

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Involucrin , Ivl

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody [IVRN/827] used in IHC (Paraffin sections) (IHC-P). GTX34803

GTX34803 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Monoclonal Antibody (IVRN/827)

REFERENCE

There are currently no references for Involucrin antibody [IVRN/827] (GTX34803). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Involucrin antibody [IVRN/827] (GTX34803). Be the first to share your experience with this product.
Package List Price ($)
$ 429