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Involucrin antibody [N1N2], N-term

Anti-Involucrin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116012
Anti-Involucrin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116012
Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012
Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012

Cat. No. GTX116012

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

human IVL-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Involucrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10626126

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

involucrin

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116012

GTX116012 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Involucrin(GTX116012) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Involucrin antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116012

GTX116012 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Involucrin(GTX116012) antibody at 1:200 dilution.

Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012

GTX116012 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Involucrin antibody [N1N2], N-term used in Western Blot (WB). GTX116012

GTX116012 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Involucrin antibody [N1N2], N-term (GTX116012) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169