GeneTex
  • Country / Location Selection

United States (US)

Involucrin antibody [SPM259]

Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802

Cat. No. GTX34802

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM259

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human, Dog, Pig, Monkey, Gorilla
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 0.1-0.2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified involucrin from human keratinocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IVL , involucrin , Involucrin

Cellular Localization

Cytoplasm

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802

GTX34802 IHC-P Image

IHC-P analysis of human skin tissue using GTX34802 Involucrin antibody [SPM259].

REFERENCE

There are currently no references for Involucrin antibody [SPM259] (GTX34802). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Involucrin antibody [SPM259] (GTX34802). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529