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Involucrin antibody [SPM259]

Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802

Cat No. GTX34802

Host Mouse
Clonality Monoclonal
Clone Name SPM259
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human, Dog, Pig, Monkey, Gorilla
See all Involucrin products;
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.1-0.2ug/ml for 30 minutes at RT

Note :

IHC-P
Digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified involucrin from human keratinocytes

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Involucrin,Ivl

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802

GTX34802 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with Involucrin Monoclonal Antibody (SPM259)
Package List Price ($)
$ 329