APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 0.1-0.2μg/ml for 30 min at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
We do not recommend use of this product for Mouse samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified involucrin from human keratinocytes
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
IVL , involucrin , Involucrin
Cellular Localization
Cytoplasm
Background
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802](/templates/default/js/lightbox/images/loading.gif "Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802")
|
GTX34802 IHC-P Image
IHC-P analysis of human skin tissue using GTX34802 Involucrin antibody [SPM259].
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REFERENCE
There are currently no references for Involucrin antibody [SPM259] (GTX34802). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Involucrin antibody [SPM259] (GTX34802). Be the first to share your experience with this product.
![Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802](/upload/website/prouct_img/small/GTX34802/GTX34802_20200115_IHC-P_1131_20011518_429.jpg "Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802")
![Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802](/upload/website/prouct_img/normal/GTX34802/GTX34802_20200115_IHC-P_1131_20011518_429.jpg "Anti-Involucrin antibody [SPM259] used in IHC (Paraffin sections) (IHC-P). GTX34802")