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Involucrin antibody [SY5]

Anti-Involucrin antibody [SY5] used in IHC (Paraffin sections) (IHC-P). GTX34804

Cat No. GTX34804

Host

Mouse

Clonality

Monoclonal

Clone Name

SY5

Isotype

IgG1

Application

IHC-P

Reactivity

Human, Dog, Pig, Monkey, Gorilla
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 0.1-0.2μg/ml for 30 min at RT

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC. Note than enzyme digestion is better than citrate buffer based epitope unmasking.

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
We do not recommend use of this product for Mouse.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified involucrin from human keratinocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IVL , involucrin , Involucrin

Cellular Localization

Cytoplasm

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody [SY5] used in IHC (Paraffin sections) (IHC-P). GTX34804

GTX34804 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34804 Involucrin antibody [SY5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339