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Involucrin antibody [SY5]

Anti-Involucrin antibody [SY5] used in IHC (Paraffin sections) (IHC-P). GTX34804

Cat. No. GTX34804

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SY5

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human, Dog, Pig, Monkey, Gorilla
Package
100 μg ($529)
See all Involucrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 0.1-0.2μg/ml for 30 min at RT

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37ºC. Note than enzyme digestion is better than citrate buffer based epitope unmasking.
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified involucrin from human keratinocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IVL , involucrin , Involucrin

Cellular Localization

Cytoplasm

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody [SY5] used in IHC (Paraffin sections) (IHC-P). GTX34804

GTX34804 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34804 Involucrin antibody [SY5].

REFERENCE

Clone Reference

Li Z et al. Stem Cell Res Ther 2015; 6:17 Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium.
Suarez-Farinas M et al. J Allergy Clin Immunol 2011; 127 (4):954-64.e1-4 Nonlesional atopic dermatitis skin is characterized by broad terminal differentiation defects and variable immune abnormalities.
Biedermann T et al. J Invest Dermatol 2010; 130 (8):1996-2009 Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529