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Involucrin antibody

Anti-Involucrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03271
Anti-Involucrin antibody used in Western Blot (WB). GTX03271
Anti-Involucrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03271

Cat. No. GTX03271

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from Human IVL(Accession P07476), corresponding to amino acid residues T561-K583.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

involucrin

Cellular Localization

Cytoplasm

Background

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Involucrin antibody used in IHC (Paraffin sections) (IHC-P). GTX03271

GTX03271 IHC-P Image

IHC-P analysis of mouse brain (left panel) and kidney (right panel) tissue using GTX03271 Involucrin antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-Involucrin antibody used in Western Blot (WB). GTX03271

GTX03271 WB Image

WB analysis of 293 lysate using GTX03271 Involucrin antibody. The lane on the right was treated with blocking peptide.

Anti-Involucrin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03271

GTX03271 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX03271 Involucrin antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 399