GeneTex
United States (US)

Islet 1 antibody

Orthogonal
GTX102807_42214_20160808_IHC-Fr_19011506_845.jpg
GTX102807_42214_20151106_IFA_19011506_103.jpg
GTX102807_43257_20180629_WB_Fraction_19011506_128.jpg
GTX102807_42214_20160721_WB_R_19011506_115.jpg
GTX102807_40730_IHC_R_19011506_205.jpg

Cat No. GTX102807

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

K562 whole cell extract , K562 nuclear extract , jurkat whole cell extract , jurkat nuclear extract , PC-12

Predict Reactivity

Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Islet 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Isl Lim Homeobox 1,Islet1,Isl-1,Isl1

Cellular Localization

Nucleus

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102807_42214_20160808_IHC-Fr_19011506_845.jpg

GTX102807 IHC-Fr Image

Islet 1 antibody detects Islet 1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102807_42214_20151106_IFA_19011506_103.jpg

GTX102807 ICC/IF Image

Islet 1 antibody detects Islet 1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX102807_43257_20180629_WB_Fraction_19011506_128.jpg

GTX102807 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102807_42214_20160721_WB_R_19011506_115.jpg

GTX102807 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102807_40730_IHC_R_19011506_205.jpg

GTX102807 IHC-P Image

Islet 1 antibody detects Islet 1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Islet 1 antibody (GTX102807) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169