*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
K562 whole cell extract , K562 nuclear extract , jurkat whole cell extract , jurkat nuclear extract , PC-12
Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Islet 1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Isl Lim Homeobox 1,Islet1,Isl-1,Isl1
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]
GTX102807 IHC-Fr Image
Islet 1 antibody detects Islet 1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).
GTX102807 ICC/IF Image
Islet 1 antibody detects Islet 1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX102807 WB Image
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102807 WB Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102807 IHC-P Image
Islet 1 antibody detects Islet 1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Islet 1 antibody (GTX102807) dilution: 1:100.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min