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Islet 2 antibody

Orthogonal Overexpression
Anti-Islet 2 antibody used in Western Blot (WB). GTX129375
Anti-Islet 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129375
Anti-Islet 2 antibody used in Western Blot (WB). GTX129375

Cat. No. GTX129375

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Islet 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

human ISL2-transfected 293T cells , HeLa nuclear extract

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Islet 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISL LIM homeobox 2

Cellular Localization

Nucleus

Background

Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.

Database

Research Area

DATA IMAGES

Anti-Islet 2 antibody used in Western Blot (WB). GTX129375

GTX129375 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet2 antibody (GTX129375) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Islet 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129375

GTX129375 IHC-P Image

Islet 2 antibody detects Islet 2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
Islet 2 stained by Islet 2 antibody (GTX129375) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Islet 2 antibody used in Western Blot (WB). GTX129375

GTX129375 WB Image

Overexpression

Islet2 antibody detects Islet2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human ISL2-transfected 293T cells
10 % SDS-PAGE
Islet2 antibody (GTX129375) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169