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JAK1 (phospho Tyr1022/1023) antibody

Orthogonal
Anti-JAK1 (phospho Tyr1022/1023) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25493
Anti-JAK1 (phospho Tyr1022/1023) antibody used in Western Blot (WB). GTX25493
Anti-JAK1 (phospho Tyr1022/1023) antibody used in Western Blot (WB). GTX25493

Cat. No. GTX25493

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Rabbit, Primate
Package
50 μl ($399)
See all JAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JAK1 protein that contains tyrosines 1022 and 1023. The sequence is conserved in mouse and pig.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_625940

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Janus kinase 1 , JAK1A , JAK1B , JTK3

Background

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-JAK1 (phospho Tyr1022/1023) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25493

GTX25493 ICC/IF Image

ICC/IF analysis of COLO 205 cells using GTX25493 JAK1 (phospho Tyr1022/1023) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

Anti-JAK1 (phospho Tyr1022/1023) antibody used in Western Blot (WB). GTX25493

GTX25493 WB Image

Orthogonal

WB analysis of samples using GTX25493 JAK1 (phospho Tyr1022/1023) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-JAK1 (phospho Tyr1022/1023) antibody used in Western Blot (WB). GTX25493

GTX25493 WB Image

Orthogonal

The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670

REFERENCE

Application Reference

Di Giorgio C et al. Front Oncol 2022; 12 : 939969 Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis.
Shen M et al. J Exp Clin Cancer Res 2019; 38 (1) : 149
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399