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JAK3 antibody [5H2]

Anti-JAK3 antibody [5H2] used in Flow cytometry (FACS). GTX83303
Anti-JAK3 antibody [5H2] used in Western Blot (WB). GTX83303
Anti-JAK3 antibody [5H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83303
Anti-JAK3 antibody [5H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83303

Cat. No. GTX83303

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all JAK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human JAK3 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Janus kinase 3 , JAK-3 , JAK3_HUMAN , JAKL , L-JAK , LJAK

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JAK3 antibody [5H2] used in Flow cytometry (FACS). GTX83303

GTX83303 FACS Image

FACS analysis of HeLa cells using GTX83303 JAK3 antibody [5H2].
Blue : JAK3
Red : negative control

Anti-JAK3 antibody [5H2] used in Western Blot (WB). GTX83303

GTX83303 WB Image

WB analysis of Jurkat cell lysate using GTX83303 JAK3 antibody [5H2].

Anti-JAK3 antibody [5H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83303

GTX83303 ICC/IF Image

ICC/IF analysis of HeLa (left) and HepG2 (right) cells using GTX83303 JAK3 antibody [5H2].
Green : JAK3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-JAK3 antibody [5H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83303

GTX83303 ICC/IF Image

ICC/IF analysis of 3T3-L1 cells using GTX83303 JAK3 antibody [5H2].
Green : JAK3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399